Simple buffer recirculator for electrophoresis
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چکیده
منابع مشابه
Useful buffer and gel systems for polyacrylamide gel electrophoresis.
Seven buffer and gel systems for polyacrylamide gel electrophoresis using discontinuous voltage and pH gradients (disc electrophoresis according to ORNSTEIN-UAVIS) and five systems using discontinuous voltage gradients at constant pH (according to ALLEN) have been compiled and characterized. Usefulness and applicability of the systems for clinical investigations are discussed and illustrated by...
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NEW BUFFER SYSTEM has recently been introduced for the separation of proteins (1) and hemoglobins (2) on starch gel. It differs from the conventional barbital system most frequently used heretofore in that the buffer used for the supporting medium differs from that used in the buffer vessels. For this reason the buffer system is referred to as discontinuous. The discontinuous buffer system appe...
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Isolation of cell free fetal DNA (cffDNA) from maternal serum usually leads to very low concentrations of DNA impeding further resolving through conventional methods of electrophoresis. Although several protocols have been described for capillary electrophoresis (CE) of double stranded DNA, they usually need using special polymers or coated capillaries which degrade over time. Herein, we propos...
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MOST CLINICAL LABORATORIES use paper electrophoresis for the separahon and quantitation of serum proteins. There has been increasing iiiterest in the fractionation of proteins Ill other body fluids, particularly cerebrospinal fluid. Successful paper electrophoresis requires a high protein concentration in the fluid applied to the l)POF strips, If tile total protein content of spinal fluid is no...
متن کاملBuffer capacity, ionic strength and heat dissipation in capillary electrophoresis
The composition of background electrolyte (BGE) in capillary electrophoresis (CE) is important for both selectivity and efficiency. Although the importance of pH and ionic strength predominates, attention should also be paid to specific conductivity, heat dissipation and buffering capacity. At a given pH/ionic strength combination, several buffers can in principle be used. Preference should be ...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 1979
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.260211118